Journal: Materials Today Bio

Article Title: Inhibition of gastric adenocarcinoma proliferation by WSGC@MS: Role of KEAP1/NRF2 signaling pathway and autophagy regulation

doi: 10.1016/j.mtbio.2025.101995

Figure Lengend Snippet: Transcriptomic analysis of gastric adenocarcinoma treated with WSGC@MS. Note: (A) DEGs analysis showing gene expression changes in the Treat group compared to the Control group. Red dots represent upregulated genes, and green dots represent downregulated genes (Control = 3, WSGC@MS = 3). (B) Expression level differences of FAM17B between tumor and normal samples across 33 types of tumors based on TCGA RNAseq data. Blue box plots represent normal tissues, and red box plots represent tumor tissues. (C) ROC curve assessing the accuracy of FAM117B as a prognostic marker. (D–E) Functional enrichment analysis showing significantly enriched GO and KEGG pathways among DEGs. Important enriched pathways include autophagy regulation, oxidative stress response, TOR complex, and AMPK signaling pathway. Blue indicates biological processes (BP), red indicates cellular components (CC), purple indicates molecular functions, and yellow indicates KEGG pathways. ∗Indicates p < 0.05, ∗∗indicates p < 0.01, ∗∗∗indicates p < 0.001 between the two groups. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Western Blot was performed on the lysates with antibodies against FAM117B (NBP2-47192, Novus Biologicals), NRF2 (ab62352, Abcam), and Ubiquitin (3933S, Cell Signaling Technology) following protein precipitation.

Techniques: Gene Expression, Control, Expressing, Marker, Functional Assay

Journal: Materials Today Bio

Article Title: Inhibition of gastric adenocarcinoma proliferation by WSGC@MS: Role of KEAP1/NRF2 signaling pathway and autophagy regulation

doi: 10.1016/j.mtbio.2025.101995

Figure Lengend Snippet: WSGC@MS downregulates FAM117B, reducing its competition with NRF2 for KEAP1 binding. Note: (A) mRNA levels of NRF2 in KATO III and MKN-45 cells across different groups. (B) NRF2 protein levels in KATO III and MKN-45 cells with FAM117B knockdown or overexpression, treated with or without MG132 (20 μM), detected by Western Blot. (C–D) NRF2 degradation half-life in KATO III and MKN-45 cells across different groups. (E–F) Ubiquitination levels of NRF2 in KATO III and MKN-45 cells across different groups. ∗Indicates p < 0.05, ∗∗Indicates p < 0.01, ∗∗∗Indicates p < 0.001 between the two groups; all cell experiments were repeated three times.

Article Snippet: Western Blot was performed on the lysates with antibodies against FAM117B (NBP2-47192, Novus Biologicals), NRF2 (ab62352, Abcam), and Ubiquitin (3933S, Cell Signaling Technology) following protein precipitation.

Techniques: Binding Assay, Knockdown, Over Expression, Western Blot, Ubiquitin Proteomics